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| Id: | 18979
| | Autor: | Kim, Se -Kon; Lee, Seong- Beom; Kang, Tae -Jin; Chae, Gue -Tae
| | Título: | Detection of gene mutations related with drug resistance in Mycobacterium leprae from leprosy patients using Touch-Down (TD) PCR
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| | Fonte: | s.l; s.n; 2003. 6 p. ilus, tab.
| | Resumo: | The lack of methods to identify Mycobacterium leprae with the resistance against multi-drugs quickly and specifically has hindered effective chemotherapy against M. leprae infection. To screen M. leprae with resistance against multi-drugs, the Touch-Down (TD)-PCR has been used in this study. Sequences of the folP, rpoA, B, and gyrA, B genes were analyzed for isolates of M. leprae from leprosy patients in Korea. We amplified designated region of several genes in M. leprae involved in drug resistance and could obtain the PCR products of each gene. The mutations in the particular region of folP, rpoB, and gyrB gene were certified by TD-PCR single-stranded conformational polymorphism and DNA sequencing, respectively. (AU).
| | Descritores: | DNA BACTERIANO/quim
DNA BACTERIANO/genet
Resistência Bacteriana a Múltiplas Drogas/genet
COREIA (GEOGRAFICO)
HANSENOSTATICOS/farmacol
HANSENIASE/quimioter
HANSENIASE/microbiol
MYCOBACTERIUM LEPRAE/genet
MUTACAO PUNTUAL
REACAO EM CADEIA DA POLIMERASE/métodos
POLIMORFISMO (GENETICA)
POLIMORFISMO CONFORMACIONAL DE SIMPLES FITA
ANALISE DE SEQUÊNCIA DE DNA
| | Limites: | HUMANO
SUPPORT, NON-U.S. GOV'T
| | Meio Eletrônico: | - .
| | Localização: | BR191.1; 09096/s |
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